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Rapid Legionella Test for "In-Field" Applications Print E-mail

Rapid Legionella Test - SafeOx’s rapid Legionella in-field test offers a quick and easy way to identify the presence of Legionella bacteria in water samples with out the need for laboratory testing.

"Take control - get Legionella results in only 25 minutes ..."

Using breakthrough technology our innovative test allows users to rapidly detect Legionella bacteria in water, obtaining test results in only 25 minutes instead of days. The portability, ease of use and speed of results make the SafeOx rapid Legionella test ideal for in-field applications where the speedy identification of Legionella bacteria is safety critical.

  • Fast results –  25 minutes, not days
  • Practical design for field deployment
  • Easy to use
  • Qualitative results
  • Optimise and verify that cleaning procedures are working. If they are not, you can take immediate remedial action
  • Optimise the use of biocides and disinfectants.

Each SafeOx test kit contains everything required to perform 5 Legionella tests, including full instructions, safeox-rapid-legionella-testsample collection containers and disposable pipettes.

SafeOx in-field tests are optimised for use with hot and cold water systems, spas, showers, cooling towers and industrial water systems.

  • The SafeOx test is simple to use and fast, with no interpretation required.
  • Results are easy to read and obtained within 25 minutes.
  • There are no chemicals to measure or handle.
  • The test allows immediate understanding and response to the water conditions.
  • Use can reduce the risk of outbreaks of Legionnaires’ disease, prosecution and litigation.
  • Regular use will increase confidence in your Legionella control regime.

Easy to use

Our rapid Legionella in-field test is simple and easy to use delivering results in 25 minutes. The test uses an immunochromatographic assay to detect the presence of cell surface antigens from Legionella bacteria in a sample. The presence of antigen causes the “test line” to turn red in colour as indicated in the diagram below. A “control line” is also included in the display panel, this should always turn red if the test has been performed correctly.


 Product details

Bacteria detected Legionella pneumophila serogroup 1
Detection method Lateral flow immunochromatographic assay
Lower limit of detection Approx. 105 CFU/L    (100 CFU/mL)

Tested against a wide range of waterborne bacteria, including Acinetobacter calcoaceticus, Aeromonas hydrophila subsp. hydrophila, Bacillus subtilis, Burkholderia cepacia, Citrobacter freudii, Citrobacter koseri, Escherichia coli, Enterobacter cloacae, Klebsiellaoxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Ralstonia pickettii, Raoultella terrigena, Streptococcus pyrogenes, Yersinia ruckeri. No cross reactions observed at concentrations ≤ 109 CFU/L.

Staphylococcus aureus has been observed to cross react with the test at concentrations > 108 CFU/L.

Legionella pneumophila serogroups 4 & 7 cross react with the test at concentrations > 6x109 CFU/L. The test does not react with any other L. pneumophila serogroups or L. species tested.

Test volume 100 µL                                                              Exact volume disposable pipette provided
Sample  parameters

pH range                                                          5-10

Sample temperature range                         15-45 °C (59-113 F)

Environmental operating range                  15-40 °C (59-104 F)

Free chlorine concentration                         < 5ppm

Total Alkanlinity                                              <1000 ppm

Total Hardness                                              <1000 ppm

Biocide The product has been tested with a range of common biocides and biodispersants used in Legionella control, at their normal maximum operating concentration. Active ingredients tested include: glutaraldehyde, didecyl-dimethyl-ammonium chloride, isolthazoles, dibromonitrilopropionamide, ionic and non-ionic surfactants.The test gives a false positive if used with polymeric biguanide or THPS.
Operator time <2 minutes
Development time 25 minutes
Shelf life 18 months from manufacture when stored at room temperature (<30°C) in original packaging.


Additional Legionella test kits

Alternative versions of our rapid Legionella in-field test are also available including:

  • Industrial Legionella Test - These tests are optimised for use with industrial water systems, cooling towers and whirlpool spas.
  • Legionella Biofilm Test – Allows the identification of biofilm contamination.
  • Domestic Legionella Test - Ideal for testing hot and cold water systems in domestic systems.

 Technical support and advice

If you require technical support or advice relating to our range of rapid "in-field" Legionella test kits please contact one of our specialist advisors on +44 (0) 161 877 2334 or submit your questions using our Technical Support page.